An adult thymic stromal-cell suspension model for in vitro positive selection.

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Date

1998

Type de document
Périmètre
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Source

Serveur académique Lausannois

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Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/9814589

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pissn/1044-6672

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_65932

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Serveur Académique Lausannois

Organisation

Université de Lausanne et CHUV

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info:eu-repo/semantics/openAccess , Copying allowed only for non-profit organizations , https://serval.unil.ch/disclaimer

Mots-clés 0

Animals; Antigens, CD/analysis; Antigens, Differentiation, T-Lymphocyte/analysis; CD4-Positive T-Lymphocytes/cytology; CD4-Positive T-Lymphocytes/immunology; CD8-Positive T-Lymphocytes/cytology; CD8-Positive T-Lymphocytes/immunology; Cell Differentiation; Cells, Cultured; Coculture Techniques; H-2 Antigens/immunology; Hematopoietic Stem Cells/physiology; Lymphocyte Activation; Lymphocytic choriomeningitis virus/immunology; Mice; Mice, Transgenic; Models, Immunological; Peptides/immunology; Receptors, Antigen, T-Cell, alpha-beta/immunology; Receptors, Antigen, T-Cell, alpha-beta/metabolism; Signal Transduction; Stromal Cells/immunology; T-Lymphocyte Subsets/cytology; T-Lymphocyte Subsets/immunology; Thymus Gland/cytology; Thymus Gland/growth & development

Sujets proches En

Thymus gland Pattern Model

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A.P. Chidgey et al., « An adult thymic stromal-cell suspension model for in vitro positive selection. », Serveur académique Lausannois, ID : 10670/1.mu4jif

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Presented here is a cell-suspension model for positive selection using thymocytes from alphabeta-TCR (H-2Db-restricted) transgenic mice specific to the lymphocytic choriomeningitis virus (LCMV) on a nonselecting MHC background (H-2d or TAP-1 -/-), cocultured with freshly isolated adult thymus stromal cells of the selecting MHC type. The thymic stromal cells alone induced positive selection of functional CD4- CD8+ cells whose kinetics and efficiency were enhanced by nominal peptide. Fibroblasts expressing the selecting MHC alone did not induce positive selection; however, together with nonselecting stroma and nominal peptide, there was inefficient positive. These results suggest multiple signaling in positive selection with selection events able to occur on multiple-cell types. The ease with which this model can be manipulated should greatly facilitate the resolution of the mechanisms of positive selection in normal and pathological states.

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