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23 septembre 2021
- handle: 10670/1.n6rg1i
- hal: hal-04040013
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Speciation (Biology) Deoxyribonucleic acid TNA (Nucleic acid) Desoxyribonucleic acid Thymonucleic acid Dasyprocta Chloromis Dasyproctinae AgoutiesCiter ce document
Sophie Rabinow et al., « Efficient Amplification of DNA from Ancient Caribbean Agouti (Dasyproctidae: Dasyprocta) », HAL-SHS : archéologie, ID : 10670/1.n6rg1i
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The agouti was one of the many synanthropic species humans translocated to theCaribbean islands from South America by at least AD 35. Here, we apply a geneticcommensal model to the medium-size rodent agouti (Dasyprocta sp.) in order toexplore interactions among Indigenous peoples in the Caribbean and South America.26 cytochrome b gene (Cytb) fragments (97 bp to 274 bp) were successfully amplifiedfrom 30 archaeological agouti bones from four sites: Sabazan (n = 5) and Grand Bay(n = 19) on Carriacou, Macabou (n = 1) on Martinique, and BK77 Grand Case (n = 1)on Saint Martin. The high success rate and good quality of the ancient DNA (aDNA)sequences demonstrates the potential of the modified silica-spin column method byYang et al. (1998) for extracting DNA from ancient specimens from tropical climates.Sequence analysis of the obtained fragments advance species identification ofCaribbean agouti and knowledge of population structure, providing a strong basis fortracing Indigenous mobility. This paper emphasizes the potential of the commensalmodel for the study of island interactions and suggests great promise for thecontinuation of aDNA research in the Caribbean and other tropical regions.