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1 avril 2009
- handle: 10670/1.obm3kc
Ce document est lié à :
10.4067/S0717-34582009000200012
info:eu-repo/semantics/openAccess
Mots-clés
b-Galactosidase delta milli pH differential pHSujets proches
Measuring MensurationCiter ce document
Cristian Acevedo et al., « Measuring b-Galactosidase activity at pH 6 with a differential pH sensor », Electronic Journal of Biotechnology, ID : 10670/1.obm3kc
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Résumé
The b-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure b-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.