6 août 2024
Suli-Anne Laurin et al., « Pharmacological chaperones : therapeutic potential for diseases resulting from GPCR misfolding », Papyrus : le dépôt institutionnel de l'Université de Montréal, ID : 10.1002/9781119774198.ch3
Adequate protein folding and trafficking represent crucial processes in the proper functioning of cells. Their integrity is ensured by highly regulated multi-step quality control mechanisms at different locations in the protein synthesis and secretory pathway. For membrane proteins such as G protein-coupled receptors (GPCR), folding and quality control occur mainly in the endoplasmic reticulum and the Golgi complex. Mutations in the coding sequence of GPCR often lead to incomplete or inappropriate folding that results in their recognition and their intracellular retention by the quality control system, preventing their targeting to the plasma membrane, where they usually mediate their action. Given the pleiotropic roles of GPCR in recognizing many signalling molecules(e.g., hormones, neurotransmitters, cytokines, odours, etc.), such mutations often lead to pathologies known as conformational diseases. Among them, the best characterized were retinitis pigmentosa, nephrogenic diabetes insipidus, hypogonadotropic hypogonadism and severe early-onset obesity resulting from mutation in the genes coding for rhodopsin (RHO), vasopressin type 2 receptor (V2R), gonadotropin-releasing hormone receptor (GnRHR) and melanocortin type 4 receptor (MC4R), respectively. In recent years, molecules known as chemical and pharmacological chaperones were found to promote the proper folding, trafficking and function of mutant forms of GPCR responsible for conformational diseases, thus opening avenues for the development of new treatments for these ailments. Here, we briefly review the mechanisms involved in protein folding and its quality control, and focus on the mechanism of action of GPCR-targeting pharmacological chaperones, their in vivo action, as well as their potentials and challenges for clinical development.