Fast determination of glucosamine in pharmaceutical formulations by high performance liquid chromatography without pre-column derivatization
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2014
- handle: 10670/1.pcqrwl
- 0188-6266
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Acta Universitaria
Mots-clés
Multidisciplinarias (Ciencias Sociales) Glucosamine pharmaceutical formulations reversed phase liquid chromatographySujets proches
High performance chromatography High speed liquid chromatography High sensitivity liquid chromatography High pressure liquid chromatography HPLC (Chromatography)Citer ce document
Armando Alcázar Magaña et al., « Fast determination of glucosamine in pharmaceutical formulations by high performance liquid chromatography without pre-column derivatization », Acta Universitaria, ID : 10670/1.pcqrwl
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Résumé
"The goal of this work was to achieve fast determination of glucosamine in pharmaceutical for - mulations by high performance liquid chromatography. Commercial tablets were dissolved in water and after filtration, 6 μL were injected to Luna C18 column (250 mm × 4.6 mm, 5μm). Diode Array Detector (DAD) was set at 193 nm. Using sodium perchlorate (50 mM, pH 6.5): acetonitrile (99:1, v/v) at 0.8 mL min -1 as a mobile phase, glucosamine eluted with the retention time 2.834 min ± 0.003 min and the peak purity in real sample was 998.9 ± 3.3. The detection limits evaluated for peak height and peak area measurements were 0.05 μg mL -1 and 0.02 μg mL -1 , respectively. The between-day precision relative standard deviation (RSD) evaluated at two concentration levels was always below 1%."