Afin d’obtenir de nouvelles informations sur le contexte d’enfouissement de l’enfant néandertalien nommé La Ferrassie 8, nous avons analysé des restes indéterminés du locus où le squelette humain partiel a été découvert. Notre étude porte sur 20 esquilles osseuses où la présence d’ADN de 10 espèces,...
Trichophyton indotineae causes dermatophytosis that is resistant to terbinafine and azole compounds. The aim of this study was to determine the mechanisms of resistance to itraconazole (ITC) and voriconazole (VRC) in strains of T. indotineae. Two azole-sensitive strains (ITC MIC < 0.125 μg/mL; VRC M...
In neuroblastoma (NB), the ALK receptor tyrosine kinase can be constitutively activated through activating point mutations or genomic amplification. We studied ALK genetic alterations in high-risk (HR) patients on the HR-NBL1/SIOPEN trial to determine their frequency, correlation with clinical param...
Merkel cell carcinoma (MCC) is an aggressive skin tumor with neuroendocrine differentiation, mainly affecting elderly population or immunocompromised individuals. As methylation of the human telomerase reverse transcriptase (mhTERT) has been shown to be a prognostic factor in different tumors, we in...
Epithelial ovarian cancer (EOC) is usually diagnosed at an advanced stage and significantly contributes to cancer mortality in women. Despite multimodal treatment associating chemotherapy and surgery, most patients ultimately progress and require palliative systemic therapy. In EOC, the efficacy of...
Although tick-borne pathogens have been reported as an important cause of imported fever, the incidence of Anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis (HGA), in travellers is unknown. We conducted a prospective cohort study to investigate the aetiologies of feve...
Oncogenic activation of the FGFR pathway is frequent in lung and other cancers. However, due to drug resistance, pharmacological blockage of aberrant FGFR signaling has provided little clinical benefit in patients with FGFR-amplified tumors. The determining factors for the limited efficacy of FGFR-t...
Alterations of the Retinoblastoma (Rb) pathway are frequent in ovarian cancer, typically resulting from CDKN2A down-regulation, CCNE1 amplification, CCND1/2 amplification, and RB1 loss. However, bi-allelic CDKN2A mutation or homozygous deletion is a very rare event, concerning less than 5% of patien...
The molecular alterations of pancreatic acinar cell carcinomas (ACCs) and mixed acinar-neuroendocrine carcinomas (MANECs) are not completely understood, and the possible role of c-MYC amplification in tumor development, progression, and prognosis is not known. We have investigated c-MYC gene amplifi...
Double minutes (dmin), homogeneously staining regions, and ring chromosomes are vehicles of gene amplification in cancer. The underlying mechanism leading to their formation as well as their structure and function in acute myeloid leukemia (AML) remain mysterious. We combined a range of high-resolut...
Several centuries of uninterrupted mining activities in Zacatecas, Mexico has caused soil pollution with toxic metals and metalloids such as arsenic. In this study, the arsenic-tolerance of ten bacterial isolates from a metal contaminated site were analyzed, and high tolerance was observed in both s...
"Several centuries of uninterrupted mining activities in Zacatecas, Mexico has caused soil pollution with toxic metals and metalloids such as arsenic. In this study, the arsenic-tolerance of ten bacterial isolates from a metal contaminated site were analyzed, and high tolerance was observed in both...
Chromogenic immunohistochemistry (IHC) is omnipresent in cancer diagnosis, but has also been criticized for its technical limit in quantifying the level of protein expression on tissue sections, thus potentially masking clinically relevant data. Shifting from qualitative to quantitative, immunofluor...
Background Plant tissues must be preserved in their collection state, especially for genome-wide expression profile studies. Lyophilization is a feasible, affordable tool when fresh tissues cannot be shipped at ultralow temperatures from their origin to the place of analysis. In this study, the tota...
Although anaplastic large-cell lymphomas (ALCL) carrying anaplastic lymphoma kinase (ALK) have a relatively good prognosis, aggressive forms exist. We have identified a novel translocation, causing the fusion of the TRAF1 and ALK genes, in one patient who presented with a leukemic ALK+ ALCL (ALCL-11...
La pullulation bactérienne (dysbiose) du grêle (PBG) est caractérisée par un nombre excessif de bactéries dans l’intestin grêle et une malabsorption. La symptomatologie digestive associe des troubles du transit (alternances diarrhées/constipation), des flatulences et des symptômes liés à la malabsor...
RNA extraction from mangrove tissues can be difficult due to the presence of polyphenolic and polysaccharide compounds upon cell disruption. Besides, a successful RNA isolation from mangrove tissues sometimes can be strain and species specific. Two different methods were used to extract RNA from tis...
L’herpèsvirose canine est une maladie infectieuse et contagieuse due à un alphaherpèsvirus qui entraîne des troubles de la reproduction. Ce virus peut être également évoqué dans le syndrome de la «toux de chenil » . Après contamination, l’animal subit une séroconversion et devient séropositif. Ensui...
BACKGROUND: MYCN oncogene amplification has been defined as the most important prognostic factor for neuroblastoma (NB), the most common solid extracranial neoplasm in children. High copy numbers are strongly associated with rapid tumor progression and poor outcome, independently of tumor stage or p...
Bien que connue de longue date dans plusieurs pays européens, l’ehrlichiose ovine n’a été identifiée en France qu’en avril 2006, grâce au transfert de connaissances au sein de réseaux d’épidémiosurveillance et à l’introduction de technologies modernes de diagnostic. Ces techniques d’immunofluorescen...
Les mycoplasmes pathogènes du porc ont un impact important sur la production porcine. La pneumonie induite par Mycoplasma hyopneumoniae, seul ou associé à d’autres agents infectieux, est une préoccupation majeure dans le monde entier. La culture et l’isolement de M. hyopneumoniae sont difficiles et...
Bean yellow dwarf geminivirus (BeYDV) has been used as a potential vector to improve foreign gene expression, specifically, to achieve higher yields of vaccine proteins in plants. Previously, we have shown that when the BeYDV replication initiator protein Rep was provided in trans, replication and g...
An earlier study revealed the strong phylogeographical structure of the lesser white-toothed shrew (Crocidura suaveolens group) within the northern Palaearctic. Here, we aim to reconstruct the colonization history of Mediterranean islands and to clarify the biogeography and phylogeographical relatio...
Peruchon Sandrine, Henault Sylvie, Mendy Christiane, Vaissaire Josée. Diagnostic de la tularémie par amplification génique in vitro (PCR). In: Bulletin de l'Académie Vétérinaire de France tome 159 n°2, 2006. pp. 165-168.