Identification of In Vitro Metabolites of Synthetic Phenolic Antioxidants BHT, BHA, and TBHQ by LC-HRMS/MS
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2020
- handle: 10670/1.6e2iai
- Ousji, Ons et Sleno, Lekha (2020). « Identification of In Vitro Metabolites of Synthetic Phenolic Antioxidants BHT, BHA, and TBHQ by LC-HRMS/MS ». Int. J. Mol. Sci., 21, pp. 9525-9537.
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http://archipel.uqam.ca/14459/
Ce document est lié à :
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC77651 [...]
Ce document est lié à :
doi:10.3390/ijms21249525
Mots-clés
BHT BHA synthetic antioxidant metabolites in vitro incubations liquid chromatography high-resolution tandem mass spectrometrySujets proches
Secondary metabolitesCiter ce document
Ons Ousji et al., « Identification of In Vitro Metabolites of Synthetic Phenolic Antioxidants BHT, BHA, and TBHQ by LC-HRMS/MS », UQAM Archipel : articles scientifiques, ID : 10670/1.6e2iai
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Résumé
Butylated hydroxytoluene (BHT) and its analogs, butylated hydroxyanisole (BHA) and tert-butyl-hydroquinone (TBHQ), are widely used synthetic preservatives to inhibit lipid oxidation in the food, cosmetic and pharmaceutical industries. Despite their widespread use, little is known about their human exposure and related biotransformation products. The metabolism of these compounds was investigated using in vitro incubations with human and rat liver fractions. Liquid chromatography coupled to high-resolution tandem mass spectrometry was employed to detect and characterize stable and reactive species formed via oxidative metabolism, as well as phase II conjugates. Several oxidative metabolites have been detected, as well as glutathione, glucuronide, and sulfate conjugates, many of which were not previously reported. A combination of accurate mass measurements, MS/MS fragmentation behavior, and isotope-labeling studies were used to elucidate metabolite structures.