Practical use of immobilized lysozyme for the remediation process of Escherichia coli in aqueous solution
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1 janvier 2007
- handle: 10670/1.s1gio9
Ce document est lié à :
10.4067/S0717-34582007000100015
info:eu-repo/semantics/openAccess
Mots-clés
Escherichia coli lysozyme self-assemblySujets proches
Muramidase Mucopeptide glucohydrolase E. coli (Bacterium)Citer ce document
Carlucio R Alves et al., « Practical use of immobilized lysozyme for the remediation process of Escherichia coli in aqueous solution », Electronic Journal of Biotechnology, ID : 10670/1.s1gio9
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Résumé
The lysozyme enzyme was immobilized on vitreous surface (fragments with diameters of 0.3 and 1.0 mm) for remediation of the microorganism Escherichia coli JM 109 into fresh water and saline solutions with 0.9% NaCl (w/v). Characterization of enzymatic film was carried out by infrared spectroscopy and atomic force microscopy techniques. Bactericide activity of the enzyme was evaluated by spectrophotometric analysis. It was verified that the enzymatic film was strongly coupled with the vitreous surface. The topographic analysis demonstrated that the deposited film was uniform and homogeneous. It was observed bactericide activity of film deposited on vitreous surface with 0.3 mm in fresh and saline solutions. This fact was not verified to vitreous fragments with 1.0 mm of diameter