Despite the existence of many different food preservation methods, foodborne pathogens are still the most common related problems to dairy products. Developing naturally alternative techniques to control such problems is necessary. This research was planned to assess the bacteriological quality of y...
Infectious diseases cause devastating economic losses in the poultry industry. It is important to boost factors of natural immunity to improve defense against infectious diseases in birds. The experimental groups (105 turkey poults allocated to 7 treatment groups) received basal diet plus 1% dry her...
Chlamydiales species are obligate intracellular bacteria lacking a classical peptidoglycan sacculus but relying on peptidoglycan synthesis for cytokinesis. While septal peptidoglycan biosynthesis seems to be regulated by MreB actin and its membrane anchor RodZ rather than FtsZ tubulin in Chlamydiale...
The increasing demand for long lasting antifouling surfaces has steered the development of accessible, novel, biocompatible and environmentally-friendly materials. Inspired by lubricin (LUB), a component of mammalian synovial fluid with excellent antifouling properties, we designed three block polym...
To define the bottlenecks that restrict antigen expression after oral administration of viral-vectored vaccines, we tracked vectors derived from the human adenovirus type 5 at whole body, tissue, and cellular scales throughout the digestive tract in a murine model of oral delivery. After intragastri...
Στο παρόν άρθρο ανασκοπείται η σχετική με τους αμυντικούς μηχανισμούς του μαστικού αδένα των αγελάδων, βιβλιογραφία. Το άρθρο διαρθρώνεται σε τρεις ενότητες: (i) Η θηλή, (ϋ) Κυτταρικοί αμυντικοί μηχανισμοί (αριθμός σωματικών κυττάρων,τύπος σωματικών κυττάρων, μέτρηση σωματικών κυττάρων,αμυντικός ρόλ...
Polymers made of poly(ethylene glycol) chains grafted to poly(lactic acid) chains (PEG-g-PLA) were used to produce stealth drug nanocarriers. A library of comb-like PEG-g-PLA polymers with different PEG grafting densities was prepared in order to obtain nanocarriers with dense PEG brushes at their s...
Background Lysozyme plays a crucial role in innate immunity with its well-recognized bacteriolytic activity. In this study, the influence of expression parameters (inoculation volume, culture volume, growth time, induction temperature and time, initial pH and methanol concentration) on human lysozym...
Background The sea cucumber lysozyme belongs to the family of invertebrate lysozymes and is thought to be a key defense factor in protecting aquaculture animals against bacterial infection. Recently, evidence was found that the sea cucumber lysozyme exerts broad spectrum antimicrobial action in vitr...
Background: Recombinant proteins, including antibodies and antibody fragments, often contain disulfide bond bridges that are necessary for their folding, stability and function. Production of disulfide-bond-containing proteins in the periplasm of Escherichia coli has been very useful, due to unique...
The chicken-type lysozyme of the insect Spodoptera litura (SLLyz) is a polypeptide of 121 amino acids containing four disulfide bridges and 17 rare codons and participates in innate defense as an anti-bacterial enzyme. The recombinant S. litura lysozyme (rSLLyz) expressed as a C-terminal fusion prot...
B lymphocytes are considered to play a minimal role in host defense against Leishmania major. In this study, the contribution of B cells to susceptibility to infection with different strains of L. major was investigated in BALB/c mice lacking mature B cells due to the disruption of the IgM transmemb...
The lysozyme enzyme was immobilized on vitreous surface (fragments with diameters of 0.3 and 1.0 mm) for remediation of the microorganism Escherichia coli JM 109 into fresh water and saline solutions with 0.9% NaCl (w/v). Characterization of enzymatic film was carried out by infrared spectroscopy an...
Shrimp Lysozyme (Lyz) is a key component of the antibacterial response as part of the innate defense in Crustacea; however, it has not been possible to purify this protein because of the very low amount present in the shrimp blood cells (hemocytes). In an effort to produce enough protein to study it...
"Se evaluó las respuestas inmunológicas y de sistemas enzimáticosantioxidantes que participan en el control de toxicidad deoxidoradicales en la ostra perla Pinctada imbricata, después de laexposición aguda (7d) a 25 y 100% de la fracción soluble de FuelOil Nº6 (FSA), una fuente de hidrocarburos poli...
The protein lysozyme was deposited onto a permeable support comprising chemically functionalized glass fiber. The main objective of this study was to set a stable organic net with no effect on the medium bed permeability and a preliminary test the activity of this enzyme under immobilized conditions...
Transition de phase dans un cristal de protéine : l'exemple du lysozyme. Formation, à partir d'une solution, et dans les mêmes conditions expérimentales, de cristaux de lysozymes soit quadratiques (température 25 °) ainsi que la transition de phase dans le cristal de protéine provoquée par la tempér...
Les auteurs mettent en évidence la production de radicaux libres dans des protéines par action photodynamique. L'analyse systématique de ce phénomène, dans le cas de la gélatine, delatrypsine, delapepsine et du lysozyme, photosensibilisés, en présence d'oxygène, par la proflavine, révèle que les rad...
Le rôle de la cristallographie des protéines est double : déterminer l'architecture moléculaire de la protéine et aider à définir la fonction de la molécule. La détermination de la structure d'une protéine se fait par la méthode du remplacement isomorphique. Il est possible de préparer des dérivés d...